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Orbit, a versatile image analysis software for biological image-based quantification
/*
* Orbit, a versatile image analysis software for biological image-based quantification.
* Copyright (C) 2009 - 2018 Idorsia Pharmaceuticals Ltd., Hegenheimermattweg 91, CH-4123 Allschwil, Switzerland.
*
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see .
*
*/
package com.actelion.research.orbit.imageAnalysis.components;
import com.actelion.research.orbit.beans.RawMeta;
import com.actelion.research.orbit.dal.IOrbitImage;
import com.actelion.research.orbit.dal.IOrbitImageMultiChannel;
import com.actelion.research.orbit.imageAnalysis.dal.DALConfig;
import com.actelion.research.orbit.imageAnalysis.utils.OrbitTiledImage2;
import com.actelion.research.orbit.imageAnalysis.utils.OrbitTiledImageIOrbitImage;
import com.actelion.research.orbit.imageAnalysis.utils.OrbitUtils;
import com.actelion.research.orbit.utils.ChannelToHue;
import com.actelion.research.orbit.utils.RawMetaFactoryFile;
import com.actelion.research.orbit.utils.RawUtilsCommon;
import imageJ.Colour_Deconvolution;
import org.slf4j.Logger;
import org.slf4j.LoggerFactory;
import javax.swing.*;
import javax.swing.event.ChangeEvent;
import javax.swing.event.ChangeListener;
import java.awt.*;
import java.awt.event.*;
import java.util.ArrayList;
import java.util.Arrays;
import java.util.Date;
import java.util.List;
import java.util.prefs.Preferences;
public class AdjustPanel extends JPanel {
private static final long serialVersionUID = 1L;
private final static Logger logger = LoggerFactory.getLogger(AdjustPanel.class);
private final DoubleClickSlider brightnessSlider = new DoubleClickSlider(-100, 100, 0);
private final DoubleClickSlider gammaSlider = new DoubleClickSlider(0, 320, 100);
private final DoubleClickSlider contrastSlider = new DoubleClickSlider(0, 800, 100);
private final DoubleClickSlider blurSlider = new DoubleClickSlider(0, 50, 0);
private final DoubleClickSlider redSlider = new DoubleClickSlider(-100, 100, 0);
private final DoubleClickSlider greenSlider = new DoubleClickSlider(-100, 100, 0);
private final DoubleClickSlider blueSlider = new DoubleClickSlider(-100, 100, 0);
private final JCheckBox redCb = new JCheckBox("red ", true);
private final JCheckBox greenCb = new JCheckBox("green", true);
private final JCheckBox blueCb = new JCheckBox("blue ", true);
private final JPanel multiChannelPanel = new JPanel();
private final JComboBox deconvCb = new JComboBox(Colour_Deconvolution.stainings);
private final JRadioButton deconvChannel0 = new JRadioButton("Disable", true);
private final JRadioButton deconvChannel1 = new JRadioButton("Stain 1", false);
private final JRadioButton deconvChannel2 = new JRadioButton("Stain 2", false);
private final JRadioButton deconvChannel3 = new JRadioButton("Comp", false);
private final JButton saveBtn = new JButton("save adjustments");
private final JButton resetBtn = new JButton("reset values");
protected final Preferences channelPrefs = Preferences.userNodeForPackage(ChannelToHue.class);
public AdjustPanel() {
brightnessSlider.setPaintLabels(true);
brightnessSlider.setPaintTicks(true);
saveBtn.addActionListener(new ActionListener() {
public void actionPerformed(ActionEvent e) {
saveAdjustments();
}
});
resetBtn.addActionListener(new ActionListener() {
public void actionPerformed(ActionEvent e) {
resetValues();
}
});
brightnessSlider.addChangeListener(new ChangeListener() {
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
int b = brightnessSlider.getValue();
iFrame.recognitionFrame.bimg.setBrightness(b);
if (!brightnessSlider.getValueIsAdjusting()) {
updateIFrame(iFrame);
}
}
}
});
contrastSlider.addChangeListener(new ChangeListener() {
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
int b = contrastSlider.getValue();
iFrame.recognitionFrame.bimg.setContrast(b);
if (!contrastSlider.getValueIsAdjusting()) {
updateIFrame(iFrame);
}
}
}
});
gammaSlider.addChangeListener(new ChangeListener() {
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
int b = gammaSlider.getValue();
iFrame.recognitionFrame.bimg.setGamma(b);
if (!gammaSlider.getValueIsAdjusting()) {
updateIFrame(iFrame);
}
}
}
});
blurSlider.addChangeListener(new ChangeListener() {
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
int b = blurSlider.getValue();
iFrame.recognitionFrame.bimg.setBlur(b);
if (!blurSlider.getValueIsAdjusting()) {
updateIFrame(iFrame);
}
}
}
});
ChangeListener rgbChangeListener = new ChangeListener() {
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
int r = redSlider.getValue();
int g = greenSlider.getValue();
int b = blueSlider.getValue();
iFrame.recognitionFrame.bimg.setRedAdjust(r);
iFrame.recognitionFrame.bimg.setGreenAdjust(g);
iFrame.recognitionFrame.bimg.setBlueAdjust(b);
iFrame.recognitionFrame.bimg.setRedActive(redCb.isSelected());
iFrame.recognitionFrame.bimg.setGreenActive(greenCb.isSelected());
iFrame.recognitionFrame.bimg.setBlueActive(blueCb.isSelected());
if (!(redSlider.getValueIsAdjusting()||greenSlider.getValueIsAdjusting()||blueSlider.getValueIsAdjusting())) {
updateIFrame(iFrame);
}
}
}
};
redSlider.addChangeListener(rgbChangeListener);
greenSlider.addChangeListener(rgbChangeListener);
blueSlider.addChangeListener(rgbChangeListener);
redCb.addChangeListener(rgbChangeListener);
greenCb.addChangeListener(rgbChangeListener);
blueCb.addChangeListener(rgbChangeListener);
deconvChannel0.setToolTipText("disable color deconvolution");
deconvChannel0.addChangeListener(new ChangeListener() {
@Override
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
if (deconvChannel0.isSelected()) {
iFrame.recognitionFrame.bimg.setDeconvChannel(0);
updateIFrame(iFrame);
}
}
}
});
deconvChannel1.setToolTipText("select primary staining");
deconvChannel1.addChangeListener(new ChangeListener() {
@Override
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
if (deconvChannel1.isSelected() && deconvCb.getSelectedItem() != null && (!deconvCb.getSelectedItem().equals(Colour_Deconvolution.DECONV_NONE))) {
iFrame.recognitionFrame.bimg.setDeconvName((String) deconvCb.getSelectedItem());
iFrame.recognitionFrame.bimg.setDeconvChannel(1);
updateIFrame(iFrame);
}
}
}
});
deconvChannel2.setToolTipText("select secondary staining");
deconvChannel2.addChangeListener(new ChangeListener() {
@Override
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
if (deconvChannel2.isSelected() && deconvCb.getSelectedItem() != null && (!deconvCb.getSelectedItem().equals(Colour_Deconvolution.DECONV_NONE))) {
iFrame.recognitionFrame.bimg.setDeconvName((String) deconvCb.getSelectedItem());
iFrame.recognitionFrame.bimg.setDeconvChannel(2);
updateIFrame(iFrame);
}
}
}
});
deconvChannel3.setToolTipText("select complementary color to staining 1 + staining 2");
deconvChannel3.addChangeListener(new ChangeListener() {
@Override
public void stateChanged(ChangeEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
if (deconvChannel3.isSelected() && deconvCb.getSelectedItem() != null && (!deconvCb.getSelectedItem().equals(Colour_Deconvolution.DECONV_NONE))) {
iFrame.recognitionFrame.bimg.setDeconvName((String) deconvCb.getSelectedItem());
iFrame.recognitionFrame.bimg.setDeconvChannel(3);
updateIFrame(iFrame);
}
}
}
});
deconvCb.addItemListener(new ItemListener() {
@Override
public void itemStateChanged(ItemEvent e) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null) {
if (deconvCb.getSelectedItem() != null) {
String name = (String) deconvCb.getSelectedItem();
iFrame.recognitionFrame.bimg.setDeconvName(name);
if (name.equals(Colour_Deconvolution.DECONV_NONE)) {
iFrame.recognitionFrame.bimg.setDeconvChannel(0);
} else {
if (iFrame.recognitionFrame.bimg.getDeconvChannel() < 1) {
if (deconvChannel0.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(0);
if (deconvChannel1.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(1);
if (deconvChannel2.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(2);
if (deconvChannel3.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(3);
}
}
updateIFrame(iFrame);
}
}
}
});
JLabel gammaLabel = new JLabel("Gamma:");
JLabel brightnessLabel = new JLabel("Brightness:");
JLabel contrastLabel = new JLabel("Contrast:");
JLabel channelsLabel = new JLabel("RGB Channels:");
JLabel blurLabel = new JLabel("Blur:");
// layout
redCb.setForeground(Color.red);
JPanel redPanel = new JPanel(new BorderLayout());
redPanel.add(redCb, BorderLayout.WEST);
redPanel.add(DoubleClickSlider.wrapToPanelWithValue(redSlider, 0, 1), BorderLayout.CENTER);
greenCb.setForeground(new Color(20, 200, 20));
JPanel greenPanel = new JPanel(new BorderLayout());
greenPanel.add(greenCb, BorderLayout.WEST);
greenPanel.add(DoubleClickSlider.wrapToPanelWithValue(greenSlider, 0, 1), BorderLayout.CENTER);
blueCb.setForeground(Color.blue);
JPanel bluePanel = new JPanel(new BorderLayout());
bluePanel.add(blueCb, BorderLayout.WEST);
bluePanel.add(DoubleClickSlider.wrapToPanelWithValue(blueSlider, 0, 1), BorderLayout.CENTER);
JPanel radioBtnPanel = new JPanel();
radioBtnPanel.add(deconvChannel0);
radioBtnPanel.add(deconvChannel1);
radioBtnPanel.add(deconvChannel2);
radioBtnPanel.add(deconvChannel3);
ButtonGroup btnGroup = new ButtonGroup();
btnGroup.add(deconvChannel0);
btnGroup.add(deconvChannel1);
btnGroup.add(deconvChannel2);
btnGroup.add(deconvChannel3);
setLayout(new GridBagLayout());
Insets insetsL = new Insets(5, 5, 0, 5);
Insets insets = new Insets(0, 5, 0, 5);
int y = 0;
add(gammaLabel, new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 0));
add(DoubleClickSlider.wrapToPanelWithValue(gammaSlider, 0, 0.01), new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(contrastLabel, new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 0));
add(DoubleClickSlider.wrapToPanelWithValue(contrastSlider, 0, 1), new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(brightnessLabel, new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 0));
add(DoubleClickSlider.wrapToPanelWithValue(brightnessSlider, 0, 1), new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
// add(blurLabel,new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 0));
// add(DoubleClickSlider.wrapToPanelWithValue(blurSlider,0,1),new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(channelsLabel, new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 0));
add(redPanel, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(greenPanel, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(bluePanel, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
multiChannelPanel.setLayout(new BoxLayout(multiChannelPanel,BoxLayout.Y_AXIS));
multiChannelPanel.setAlignmentX(Component.LEFT_ALIGNMENT);
add(multiChannelPanel, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(new JLabel("Color Deconvolution"), new GridBagConstraints(0, y++, 1, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.NONE, insetsL, 0, 5));
add(deconvCb, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(radioBtnPanel, new GridBagConstraints(0, y++, GridBagConstraints.REMAINDER, 1, 1, 1, GridBagConstraints.NORTHWEST, GridBagConstraints.HORIZONTAL, insets, 0, 0));
add(resetBtn, new GridBagConstraints(0, y, 1, 1, 0.5, 1, GridBagConstraints.SOUTH, GridBagConstraints.HORIZONTAL, insetsL, 0, 0));
add(saveBtn, new GridBagConstraints(1, y, 1, 1, 0.5, 1, GridBagConstraints.SOUTH, GridBagConstraints.HORIZONTAL, insetsL, 0, 0));
}
private synchronized void updateIFrame(ImageFrame iFrame) {
if (iFrame != null) {
iFrame.recognitionFrame.getForceRepaint().set(true);
if (iFrame.recognitionFrame.getRenderThreadOriginal() != null && iFrame.recognitionFrame.getRenderThreadOriginal().isBufferReady()) {
iFrame.recognitionFrame.getRenderThreadOriginal().setValuesDirty(true);
iFrame.recognitionFrame.getRenderThreadOriginal().setScheduleUpdate(true);
iFrame.recognitionFrame.repaint();
}
//iFrame.adjustViewport();
else iFrame.recognitionFrame.repaint();
}
}
public void resetValues() {
SwingUtilities.invokeLater(new Runnable() {
@Override
public void run() {
multiChannelPanel.removeAll();
OrbitImageAnalysis.getInstance().getSplitPanePropLoupe().resetToPreferredSizes();
}
});
brightnessSlider.setValue(brightnessSlider.getDefValue());
contrastSlider.setValue(contrastSlider.getDefValue());
gammaSlider.setValue(contrastSlider.getDefValue());
redSlider.setValue(redSlider.getDefValue());
greenSlider.setValue(greenSlider.getDefValue());
blueSlider.setValue(blueSlider.getDefValue());
redCb.setSelected(true);
greenCb.setSelected(true);
blueCb.setSelected(true);
blurSlider.setValue(0);
deconvCb.setSelectedIndex(0);
deconvChannel0.setSelected(true);
deconvChannel1.setSelected(false);
deconvChannel2.setSelected(false);
deconvChannel3.setSelected(false);
}
public void setAdjustmentValues(int brightness, int contrast, int red, int green, int blue, int gamma, boolean redSelected, boolean greenSelected, boolean blueSelected, int deconvChannel, String deconvName, final ImageFrame iFrame) {
gammaSlider.setValue(gamma);
brightnessSlider.setValue(brightness);
contrastSlider.setValue(contrast);
redSlider.setValue(red);
greenSlider.setValue(green);
blueSlider.setValue(blue);
redCb.setSelected(redSelected);
greenCb.setSelected(greenSelected);
blueCb.setSelected(blueSelected);
deconvChannel0.setSelected(true);
if (deconvChannel == 1) deconvChannel1.setSelected(true);
if (deconvChannel == 2) deconvChannel2.setSelected(true);
if (deconvChannel == 3) deconvChannel3.setSelected(true);
deconvCb.setSelectedItem(deconvName);
loadAdjustments(iFrame); // to add fluo channels
}
/**
* imports the adjustments from a string in the format bri:;con:;r:;g;b;gamma:;deconvChan:;deconvName:
*
* @param vals
*/
public void setAdjustmentValues(String vals) {
OrbitUtils.ImageAdjustments ia = OrbitUtils.parseImageAdjustments(vals);
brightnessSlider.setValue(ia.getBrightness());
contrastSlider.setValue(ia.getContrast());
redSlider.setValue(ia.getRed());
greenSlider.setValue(ia.getGreen());
blueSlider.setValue(ia.getBlue());
gammaSlider.setValue(ia.getGamma());
redCb.setSelected(true);
greenCb.setSelected(true);
blueCb.setSelected(true);
deconvCb.setSelectedItem(ia.getDeconvName());
deconvChannel0.setSelected(true);
if (ia.getDeconvChannel() == 1) deconvChannel1.setSelected(true);
if (ia.getDeconvChannel() == 2) deconvChannel2.setSelected(true);
if (ia.getDeconvChannel() == 3) deconvChannel3.setSelected(true);
}
/**
* returns bri:;con:;r:;g;b
* for storing the string as a meta value
*
* @return
*/
private String adjustmentsToString() {
String deconvName = Colour_Deconvolution.DECONV_NONE;
if (deconvCb.getSelectedItem() != null) deconvName = (String) deconvCb.getSelectedItem();
int deconvChannel = 0;
if (deconvChannel1.isSelected()) deconvChannel = 1;
if (deconvChannel2.isSelected()) deconvChannel = 2;
if (deconvChannel3.isSelected()) deconvChannel = 3;
String channelContributions = "";
IOrbitImageMultiChannel oim = getMultiChannelImage();
if (oim!=null) {
if (oim.getChannelContributions()!=null && oim.getChannelContributions().length>0) {
for (int c=0; c0) {
s += ";channelContributions:"+channelContributions;
}
System.out.println("s: "+s);
return s;
}
private boolean saveAdjustments() {
OrbitImageAnalysis.getInstance().forceLogin();
if (OrbitImageAnalysis.loginOk) {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame != null && iFrame.getRdf() != null) {
try {
String vals = adjustmentsToString();
List rms = DALConfig.getImageProvider().LoadRawMetasByRawDataFileAndName(iFrame.getRdf().getRawDataFileId(), RawUtilsCommon.STR_META_IMAGEADJUSTMENTS);
if (rms != null && rms.size() > 0) { // update
RawMeta rm = rms.get(0);
rm.setModifyDate(new Date());
rm.setUserId(OrbitImageAnalysis.loginUser);
rm.setValue(vals);
DALConfig.getImageProvider().UpdateRawMeta(rm);
logger.debug("Image adjustments updated in database. RawMeta: " + rm);
} else { // insert
RawMetaFactoryFile rmff = new RawMetaFactoryFile(iFrame.getRdf().getRawDataFileId(), new Date(), OrbitImageAnalysis.loginUser);
RawMeta rm = rmff.createMetaStr(RawUtilsCommon.STR_META_IMAGEADJUSTMENTS, vals);
DALConfig.getImageProvider().InsertRawMeta(rm);
logger.debug("Image adjustments saved to database. RawMeta: " + rm);
}
JOptionPane.showMessageDialog(this, "Image adjustments successfully saved.", "Image Adjustments Saved", JOptionPane.INFORMATION_MESSAGE);
return true;
} catch (Exception e) {
logger.error("cannot insert or update raw meta into database", e);
return false;
}
} else {
logger.error("Cannot save image adjustment data because image is not stored in the Orbit database or no image is selected.");
return false;
}
} else {
logger.debug("Image adjustments not saved because Orbit login is not ok or canceled.");
return false;
}
}
private IOrbitImageMultiChannel getMultiChannelImage() {
ImageFrame iFrame = OrbitImageAnalysis.getInstance().getIFrame();
if (iFrame!=null) {
OrbitTiledImage2 img = iFrame.recognitionFrame.bimg.getImage();
if (img instanceof OrbitTiledImageIOrbitImage) {
IOrbitImage oi = ((OrbitTiledImageIOrbitImage) img).getOrbitImage();
if (oi instanceof IOrbitImageMultiChannel) {
final IOrbitImageMultiChannel oim = (IOrbitImageMultiChannel) oi;
return oim;
}
}
}
return null;
}
private List getMultiChannelImages() {
List images = new ArrayList<>();
List iFrames = OrbitImageAnalysis.getInstance().getIFrames();
if (iFrames!=null) {
for (ImageFrame iFrame : iFrames) {
OrbitTiledImage2 img = iFrame.recognitionFrame.bimg.getImage();
if (img instanceof OrbitTiledImageIOrbitImage) {
IOrbitImage oi = ((OrbitTiledImageIOrbitImage) img).getOrbitImage();
if (oi instanceof IOrbitImageMultiChannel) {
final IOrbitImageMultiChannel oim = (IOrbitImageMultiChannel) oi;
images.add(oim);
}
}
}
}
return images;
}
public boolean loadAdjustments(final ImageFrame iFrame) {
boolean adjustmentsSet = false;
// other adjustments
if (iFrame != null && iFrame.getRdf() != null) {
try {
List rms = DALConfig.getImageProvider().LoadRawMetasByRawDataFileAndName(iFrame.getRdf().getRawDataFileId(), RawUtilsCommon.STR_META_IMAGEADJUSTMENTS);
if (rms != null && rms.size() > 0) {
RawMeta rm = rms.get(0);
setAdjustmentValues(rm.getValue());
logger.debug("Image adjustments loaded. RawMeta: " + rm);
iFrame.recognitionFrame.bimg.setBrightness(brightnessSlider.getValue());
iFrame.recognitionFrame.bimg.setContrast(contrastSlider.getValue());
iFrame.recognitionFrame.bimg.setRedAdjust(redSlider.getValue());
iFrame.recognitionFrame.bimg.setGreenAdjust(greenSlider.getValue());
iFrame.recognitionFrame.bimg.setBlueAdjust(blueSlider.getValue());
iFrame.recognitionFrame.bimg.setGamma(gammaSlider.getValue());
if (deconvCb.getSelectedItem() != null)
iFrame.recognitionFrame.bimg.setDeconvName((String) deconvCb.getSelectedItem());
if (deconvChannel0.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(0);
if (deconvChannel1.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(1);
if (deconvChannel2.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(2);
if (deconvChannel3.isSelected()) iFrame.recognitionFrame.bimg.setDeconvChannel(3);
// channel contributions
OrbitUtils.ImageAdjustments ia = OrbitUtils.parseImageAdjustments(rm.getValue());
if (ia!=null) {
IOrbitImageMultiChannel oim = getMultiChannelImage();
if (!iFrame.isChannelContributionsLoaded() && oim!=null) {
iFrame.setChannelContributionsLoaded(true);
oim.setChannelContributions(ia.getChannelContributions());
}
}
} else adjustmentsSet = false;
adjustmentsSet = true;
} catch (Exception e) {
logger.error("Cannot load image adjustment meta data from database", e);
}
}
// add multichannel components
if (iFrame!=null) {
OrbitTiledImage2 img = iFrame.recognitionFrame.bimg.getImage();
if (img instanceof OrbitTiledImageIOrbitImage) {
IOrbitImage oi = ((OrbitTiledImageIOrbitImage) img).getOrbitImage();
if (oi instanceof IOrbitImageMultiChannel) {
final IOrbitImageMultiChannel oim = (IOrbitImageMultiChannel) oi;
if (oim.getChannelNames()!=null && oim.getChannelNames().length>0) {
SwingUtilities.invokeLater(new Runnable() {
@Override
public void run() {
multiChannelPanel.removeAll();
JLabel fluoLabel = new JLabel("Fluorescence Channels:");
Box b1 = Box.createHorizontalBox();
b1.setBorder(BorderFactory.createEmptyBorder(13,0,7,0));
b1.add(fluoLabel);
b1.add(Box.createHorizontalGlue());
multiChannelPanel.add(b1);
JPanel cp = new JPanel(new GridBagLayout());
Insets insetsL = new Insets(5, 5, 0, 5);
Insets insetsCP = new Insets(6, 5, 0, 5);
Insets insets = new Insets(0, 5, 0, 5);
int y = 0;
final float multiplier = 0.01f;
for (int c=0; c