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Orbit, a versatile image analysis software for biological image-based quantification
/*
* Orbit, a versatile image analysis software for biological image-based quantification.
* Copyright (C) 2009 - 2018 Idorsia Pharmaceuticals Ltd., Hegenheimermattweg 91, CH-4123 Allschwil, Switzerland.
*
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see .
*
*/
package com.actelion.research.orbit.imageAnalysis.cp;
import ch.qos.logback.classic.Level;
import com.actelion.research.orbit.beans.RawDataFile;
import com.actelion.research.orbit.imageAnalysis.models.OrbitModel;
import com.actelion.research.orbit.imageAnalysis.models.TaskResult;
import com.actelion.research.orbit.imageAnalysis.tasks.OrbitWorker;
import org.slf4j.Logger;
import org.slf4j.LoggerFactory;
import java.io.BufferedReader;
import java.io.File;
import java.io.FileReader;
import java.util.List;
/**
* Cell Profiler pipeline
*/
public class CpStarter extends OrbitWorker {
private static final Logger log = LoggerFactory.getLogger(CpStarter.class);
private List rdfList;
private OrbitModel exclModel;
private String pipeline;
private String workDir;
private String cpExe;
private boolean storeAnnotations;
private String orbitUser = "";
public CpStarter(String orbitUser) {
this.orbitUser = orbitUser;
}
public CpStarter(String cpExe, OrbitModel exclModel, String pipeline, List rdfList, String workDir, boolean storeAnnotations, String orbitUser) {
this.cpExe = cpExe;
this.exclModel = exclModel;
this.pipeline = pipeline;
this.rdfList = rdfList;
this.workDir = workDir;
this.storeAnnotations = storeAnnotations;
this.orbitUser = orbitUser;
}
@Override
protected void doWork() throws Exception {
System.out.println("rdfList size: " + rdfList.size());
System.out.println("exclusion model: " + exclModel);
System.out.println("pipeline: " + pipeline);
System.out.println("working directory: " + workDir);
log.info("download tiles start");
CpExportTiles exportTiles = new CpExportTiles();
exportTiles.downloadTiles(rdfList, workDir, exclModel);
log.info("download tiles end");
setProgress(30);
log.info("cp executor start");
CpExecutor executor = new CpExecutor();
if (cpExe != null) executor.setCpExe(cpExe);
executor.startAllDirs(new File(workDir), pipeline);
log.info("cp executor end");
setProgress(70);
log.info("cp parser start");
CpParser cpParser = new CpParser(orbitUser);
cpParser.processObjects(workDir, pipeline, exclModel, storeAnnotations);
log.info("cp parser end");
setProgress(98);
StringBuilder sb = new StringBuilder();
try (BufferedReader inStream = new BufferedReader(new FileReader(workDir + File.separator + "objectcounts.csv"))) {
String line;
while ((line = inStream.readLine()) != null) {
sb.append(line + "\n");
}
}
taskResult = new TaskResult("Cell Profiler Results", sb.toString());
setProgress(100);
}
/**
* cpExe can be null
*
* @param searchStr
* @param pipeline
* @param workDir
* @param cpExe
*/
public void start(String searchStr, String exclModel, String pipeline, String workDir, String cpExe) throws Exception {
System.out.println("searchStr: " + searchStr);
System.out.println("exclusion model: " + exclModel);
System.out.println("pipeline: " + pipeline);
System.out.println("working directory: " + workDir);
log.info("download tiles start");
CpExportTiles exportTiles = new CpExportTiles();
exportTiles.downloadTiles(searchStr, workDir, exclModel);
log.info("download tiles end");
log.info("cp executor start");
CpExecutor executor = new CpExecutor();
if (cpExe != null) executor.setCpExe(cpExe);
executor.startAllDirs(new File(workDir), pipeline);
log.info("cp executor end");
log.info("cp parser start");
CpParser cpParser = new CpParser(orbitUser);
cpParser.processObjects(workDir, pipeline, exclModel, true);
log.info("cp parser end");
}
public static void main(String[] args) throws Exception {
Logger root = LoggerFactory.getLogger(CpStarter.class);
if (root instanceof ch.qos.logback.classic.Logger) // can only set if logback implementation
((ch.qos.logback.classic.Logger) root).setLevel(Level.TRACE);
System.out.println("CpStarter ");
if (args == null || args.length < 4) throw new IllegalArgumentException("wrong number of arguments");
String searchStr = args[0];
String exclModel = args[1];
String pipeline = args[2];
String workDir = args[3];
CpStarter starter = new CpStarter("root");
starter.start(searchStr, exclModel, pipeline, workDir, null);
}
}