dagr.tasks.picard.MergeBamAlignment.scala Maven / Gradle / Ivy
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A set of example dagr tasks.
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/*
* The MIT License
*
* Copyright (c) 2015 Fulcrum Genomics LLC
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package dagr.tasks.picard
import dagr.core.execsystem.{Cores, Memory}
import dagr.core.tasksystem.Pipe
import dagr.tasks.DataTypes.SamOrBam
import dagr.tasks.DagrDef
import DagrDef.{PathToBam, PathToFasta}
import htsjdk.samtools.SAMFileHeader.SortOrder
import htsjdk.samtools.SamPairUtil.PairOrientation
import scala.collection.mutable.ListBuffer
class MergeBamAlignment(unmapped: PathToBam,
mapped: PathToBam,
out: PathToBam,
ref: PathToFasta,
programGroupId: Option[String] = None,
programGroupVersion: Option[String] = None,
programGroupCommand: Option[String] = None,
programGroupName: Option[String] = None,
attributesToRetain: List[String] = List[String]("X0", "ZS", "ZI", "ZM", "ZC", "ZN"),
attributesToReverse: List[String] = List[String]("ad", "bd", "cd", "ae", "be", "ce"), // fgbio consensus tags
attributesToReverseComplement: List[String] = Nil,
orientation: PairOrientation = PairOrientation.FR,
maxGaps: Int = -1,
sortOrder: SortOrder = SortOrder.coordinate,
useAlignerProperPairFlags: Boolean = false
)
extends PicardTask with Pipe[SamOrBam,SamOrBam] {
requires(Cores(1), Memory("4g"))
override protected def addPicardArgs(buffer: ListBuffer[Any]): Unit = {
buffer.append("UNMAPPED=" + unmapped)
buffer.append("ALIGNED=" + mapped)
buffer.append("O=" + out)
buffer.append("R=" + ref)
buffer.append("CLIP_ADAPTERS=false")
programGroupId.foreach(pg => buffer.append("PG=" + pg))
programGroupVersion.foreach(version => buffer.append("PG_VERSION=" + version))
programGroupCommand.foreach(cmd => buffer.append("PG_COMMAND=\'" + cmd + "\'"))
programGroupName.foreach(name => buffer.append("PG_NAME=" + name))
attributesToRetain.foreach(attr => buffer.append("ATTRIBUTES_TO_RETAIN=" + attr))
attributesToReverse.foreach(attr => buffer.append("RV=" + attr))
attributesToReverseComplement.foreach(attr => buffer.append("RC=" + attr))
buffer.append("ORIENTATIONS=" + orientation.name())
buffer.append("MAX_GAPS=" + maxGaps)
buffer.append("SO=" + sortOrder.name())
buffer.append("ALIGNER_PROPER_PAIR_FLAGS=" + useAlignerProperPairFlags)
}
}