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com.fulcrumgenomics.bam.SortBam.scala Maven / Gradle / Ivy
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/*
* The MIT License
*
* Copyright (c) 2017 Fulcrum Genomics LLC
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package com.fulcrumgenomics.bam
import com.fulcrumgenomics.FgBioDef._
import com.fulcrumgenomics.bam.api.{SamOrder, SamSource, SamWriter}
import com.fulcrumgenomics.cmdline.{ClpGroups, FgBioTool}
import com.fulcrumgenomics.commons.util.LazyLogging
import com.fulcrumgenomics.sopt.{arg, clp}
import com.fulcrumgenomics.util.Io
@clp(group=ClpGroups.SamOrBam, description =
"""
|Sorts a SAM or BAM file. Several sort orders are available:
|
|1. **Coordinate**: sorts reads by their reference sequence and left-most aligned coordinate
|2. **Queryname**: sort the reads by their query (i.e. read) name
|3. **Random**: sorts the reads into a random order. The output is deterministic for any given input.
|and several
|4. **RandomQuery**: sorts the reads into a random order but keeps reads with the same
| queryname together. The ordering is deterministic for any given input.
|5. **TemplateCoordinate**: The sort order used by `GroupReadByUmi`. Sorts reads by
| the earlier unclipped 5' coordinate of the read pair, the higher unclipped 5' coordinate of the
| read pair, library, the molecular identifier (MI tag), read name, and if R1 has the lower
| coordinates of the pair.
|
|Uses a temporary directory to buffer sets of sorted reads to disk. The number of reads kept in memory
|affects memory use and can be changed with the `--max-records-in-ram` option. The temporary directory
|to use can be set with the fgbio global option `--tmp-dir`.
|
|An example invocation might look like:
|
|```bash
|java -Xmx4g -jar fgbio.jar --tmp-dir=/my/big/scratch/volume \
| SortBam --input=queryname.bam --sort-order=Coordinate --output coordinate.bam
|```
""")
class SortBam
( @arg(flag='i', doc="Input SAM or BAM.") val input: PathToBam,
@arg(flag='o', doc="Output SAM or BAM.") val output: PathToBam,
@arg(flag='s', doc="Order into which to sort the records.") val sortOrder: SamOrder = SamOrder.Coordinate,
@arg(flag='m', doc="Max records in RAM.") val maxRecordsInRam: Int = SamWriter.DefaultMaxRecordsInRam
) extends FgBioTool with LazyLogging {
Io.assertReadable(input)
Io.assertCanWriteFile(output)
override def execute(): Unit = {
val in = SamSource(input)
val out = SamWriter(output, in.header.clone(), sort=Some(sortOrder), maxRecordsInRam=maxRecordsInRam)
out ++= in
out.close()
in.safelyClose()
}
}
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