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com.fulcrumgenomics.fasta.HardMaskFasta.scala Maven / Gradle / Ivy
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/*
* The MIT License
*
* Copyright (c) 2016 Fulcrum Genomics LLC
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package com.fulcrumgenomics.fasta
import com.fulcrumgenomics.cmdline.{ClpGroups, FgBioTool}
import com.fulcrumgenomics.util.{Io, ProgressLogger}
import com.fulcrumgenomics.commons.CommonsDef._
import com.fulcrumgenomics.commons.util.LazyLogging
import com.fulcrumgenomics.sopt._
import htsjdk.samtools.reference.{ReferenceSequenceFileFactory, ReferenceSequenceFileWalker}
/**
* Tool to take in a FASTA file and convert soft-masked (i.e. lower-case) sequence to hard-masked
* sequence (i.e. Ns), for tools that cannot correctly interpret soft-masking.
*
* @author Tim Fennell
*/
@clp(description =
"""
|Converts soft-masked sequence to hard-masked in a FASTA file. All lower case bases are
|converted to Ns, all other bases are left unchanged. Line lengths are also standardized
|to allow easy indexing with `samtools faidx`"
""",
group = ClpGroups.Fasta)
class HardMaskFasta
( @arg(flag='i', doc="Input FASTA file.") val input: PathToFasta,
@arg(flag='o', doc="Output FASTA file.") val output: PathToFasta,
@arg(flag='l', doc="Line length or sequence lines.") val lineLength: Int = 100
) extends FgBioTool with LazyLogging {
override def execute(): Unit = {
Io.assertReadable(input)
Io.assertCanWriteFile(output)
val out = Io.toWriter(output)
val progress = new ProgressLogger(logger, noun="sequences", verb="masked", unit=5000)
for (seq <- ReferenceSequenceIterator(input)) {
out.append('>').append(seq.getName).append('\n')
seq.getBases.grouped(lineLength).foreach(bs => {
bs.indices.foreach(i => if (bs(i).toChar.isLower) bs(i) = 'N')
bs.foreach(out.write(_))
out.newLine()
})
progress.record()
}
out.close()
}
}
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