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/*
* The MIT License
*
* Copyright (c) 2016 The Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package picard.sam.markduplicates;
import htsjdk.samtools.util.Histogram;
import picard.sam.markduplicates.util.OpticalDuplicateFinder;
import java.util.ArrayList;
import java.util.List;
import static picard.sam.markduplicates.EstimateLibraryComplexity.PairedReadSequence;
import static picard.sam.markduplicates.EstimateLibraryComplexity.PairedReadSequenceWithBarcodes;
/**
* Algorithm for search duplicates is used in EstimateLibraryComplexity only.
* It finds duplicates comparing bases in pairs.
*
* * @author [email protected], EPAM Systems, Inc.
*/
class ElcIdenticalBasesDuplicatesFinder extends ElcDuplicatesFinder {
private boolean useBarcodes;
ElcIdenticalBasesDuplicatesFinder(double maxDiffRate, int maxReadLength, int minIdenticalBases,
boolean useBarcodes, OpticalDuplicateFinder opticalDuplicateFinder) {
super(maxDiffRate, maxReadLength, minIdenticalBases, opticalDuplicateFinder);
this.useBarcodes = useBarcodes;
}
/**
* Search duplicates in library size < BOUNDARY_LIBRARY_SIZE or reads with barcodes
*/
@Override
void searchDuplicates(List sequences,
Histogram duplicationHisto, Histogram opticalHisto) {
for (int i = 0; i < sequences.size(); ++i) {
final PairedReadSequence lhs = sequences.get(i);
if (lhs == null) continue;
final List dupes = new ArrayList<>();
for (int j = i + 1; j < sequences.size(); ++j) {
final PairedReadSequence rhs = sequences.get(j);
if (rhs == null) continue;
if (matches(lhs, rhs, maxDiffRate, useBarcodes)) {
dupes.add(rhs);
sequences.set(j, null);
}
}
fillHistogram(duplicationHisto, opticalHisto, lhs, dupes);
}
}
/**
* Checks to see if two reads pairs have sequence that are the same, give or take a few
* errors/diffs as dictated by the maxDiffRate.
*/
private boolean matches(final PairedReadSequence lhs, final PairedReadSequence rhs, final double maxDiffRate, final boolean useBarcodes) {
final int read1Length = minLength(lhs.read1, rhs.read1);
final int read2Length = minLength(lhs.read2, rhs.read2);
final int maxErrors = (int) Math.floor((read1Length + read2Length) * maxDiffRate);
int errors = 0;
if (useBarcodes) {
final PairedReadSequenceWithBarcodes lhsWithBarcodes = (PairedReadSequenceWithBarcodes) lhs;
final PairedReadSequenceWithBarcodes rhsWithBarcodes = (PairedReadSequenceWithBarcodes) rhs;
if (lhsWithBarcodes.barcode != rhsWithBarcodes.barcode ||
lhsWithBarcodes.readOneBarcode != rhsWithBarcodes.readOneBarcode ||
lhsWithBarcodes.readTwoBarcode != rhsWithBarcodes.readTwoBarcode) {
return false;
}
}
// The loop can start from MIN_IDENTICAL_BASES because we've already confirmed that
// at least those first few bases are identical when sorting.
for (int i = minIdenticalBases; i < read1Length; ++i) {
if (lhs.read1[i] != rhs.read1[i] && ++errors > maxErrors) {
return false;
}
}
for (int i = minIdenticalBases; i < read2Length; ++i) {
if (lhs.read2[i] != rhs.read2[i] && ++errors > maxErrors) {
return false;
}
}
return true;
}
}