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A Java API for high-throughput sequencing data (HTS) formats
/*
* The MIT License
*
* Copyright (c) 2015 The Broad Institute
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
* THE SOFTWARE.
*/
package htsjdk.samtools;
import htsjdk.samtools.util.CloseableIterator;
/**
* Abstract base class for all DownsamplingIterators that provides a uniform interface for recording
* and reporting statistics bout how many records have been kept and discarded.
*
* A DownsamplingIterator is an iterator that takes another iterator of SAMRecords and filters out a
* subset of those records in a random way, while ensuring that all records for a template (i.e. record name)
* are either retained or discarded. Strictly speaking the proportion parameter applies to templates,
* though in most instances it is safe to think about it being applied to records.
*
* @author Tim Fennell
*/
public abstract class DownsamplingIterator implements CloseableIterator {
private long recordsSeen;
private long recordsAccepted;
private double targetProportion;
/** Constructs a downsampling iterator that aims to retain the targetProportion of reads. */
public DownsamplingIterator(final double targetProportion) {
if (targetProportion < 0) throw new IllegalArgumentException("targetProportion must be >= 0");
if (targetProportion > 1) throw new IllegalArgumentException("targetProportion must be <= 1");
this.targetProportion = targetProportion;
}
/** Does nothing. */
@Override public void close() { /** No Op. */ }
/** Returns the number of records seen, including accepted and discarded, since creation of the last call to resetStatistics. */
public long getSeenCount() { return this.recordsSeen; }
/** Returns the number of records returned since creation of the last call to resetStatistics. */
public long getAcceptedCount() { return this.recordsAccepted; }
/** Returns the number of records discarded since creation of the last call to resetStatistics. */
public long getDiscardedCount() { return this.recordsSeen - this.recordsAccepted; }
/** Gets the fraction of records discarded since creation or the last call to resetStatistics(). */
public double getDiscardedFraction() { return getDiscardedCount() / (double) getSeenCount(); }
/** Gets the fraction of records accepted since creation or the last call to resetStatistics(). */
public double getAcceptedFraction() { return getAcceptedCount() / (double) getSeenCount(); }
/** Resets the statistics for records seen/accepted/discarded. */
public void resetStatistics() {
this.recordsSeen = 0;
this.recordsAccepted = 0;
}
/** Gets the target proportion of records that should be retained during downsampling. */
public double getTargetProportion() {
return targetProportion;
}
/** Method for subclasses to record a record as being discarded. */
protected final void recordDiscardedRecord() { this.recordsSeen++; }
/**
* Method for subclasses to record a specific record as being accepted. Null may be passed if a record
* was discarded but access to the object is no longer available.
*/
protected final void recordAcceptedRecord() { this.recordsSeen++; this.recordsAccepted++; }
/** Record one or more records as having been discarded. */
protected final void recordDiscardRecords(final long n) {
this.recordsSeen += n;
}
/** Record one or more records as having been discarded. */
protected final void recordAcceptedRecords(final long n) {
this.recordsSeen += n;
this.recordsAccepted += n;
}
/**
* Indicates whether or not the strategy implemented by this DownsamplingIterator makes any effort to
* increase accuracy beyond random sampling (i.e. to reduce the delta between the requested proportion
* of reads and the actually emitted proportion of reads).
*/
public boolean isHigherAccuracy() {
return false;
}
/** Not supported. */
@Override public void remove() {
throw new UnsupportedOperationException("remove() not supported in DownsamplingIterators");
}
}
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