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ModularImageAnalysis (MIA) is an ImageJ plugin which provides a modular framework for assembling image and object analysis workflows. Detected objects can be transformed, filtered, measured and related. Analysis workflows are batch-enabled by default, allowing easy processing of high-content datasets.

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package io.github.mianalysis.mia.module.objects.process;

import org.scijava.Priority;
import org.scijava.plugin.Plugin;

import ij.gui.Roi;
import io.github.mianalysis.mia.module.Categories;
import io.github.mianalysis.mia.module.Category;
import io.github.mianalysis.mia.module.Module;
import io.github.mianalysis.mia.module.Modules;
import io.github.mianalysis.mia.object.Obj;
import io.github.mianalysis.mia.object.Objs;
import io.github.mianalysis.mia.object.Workspace;
import io.github.mianalysis.mia.object.coordinates.volume.PointOutOfRangeException;
import io.github.mianalysis.mia.object.coordinates.volume.VolumeType;
import io.github.mianalysis.mia.object.parameters.InputObjectsP;
import io.github.mianalysis.mia.object.parameters.Parameters;
import io.github.mianalysis.mia.object.parameters.SeparatorP;
import io.github.mianalysis.mia.object.parameters.objects.OutputObjectsP;
import io.github.mianalysis.mia.object.refs.collections.ImageMeasurementRefs;
import io.github.mianalysis.mia.object.refs.collections.MetadataRefs;
import io.github.mianalysis.mia.object.refs.collections.ObjMeasurementRefs;
import io.github.mianalysis.mia.object.refs.collections.ObjMetadataRefs;
import io.github.mianalysis.mia.object.refs.collections.ParentChildRefs;
import io.github.mianalysis.mia.object.refs.collections.PartnerRefs;
import io.github.mianalysis.mia.object.system.Status;


/**
* Fit 2D convex hull to a 2D object.  If objects are in 3D, a convex hull is fit slice-by-slice.

Uses the ImageJ "Fit convex hull" function. */ @Plugin(type = Module.class, priority = Priority.LOW, visible = true) public class FitConvexHull2D extends Module { /** * */ public static final String INPUT_SEPARATOR = "Object input/output"; /** * Input objects to create 2D convex hulls for. Each convex hull will be a child of its respective input object. */ public static final String INPUT_OBJECTS = "Input objects"; /** * Output convex hull objects will be stored in the workspace with this name. Each convex hull object will be a child of the input object it was created from. */ public static final String OUTPUT_OBJECTS = "Output objects"; public Obj processObject(Obj inputObject, Objs outputObjects) { // We have to explicitly define this, as the number of slices is 1 (potentially // unlike the input object) Obj outputObject = outputObjects.createAndAddNewObject(VolumeType.QUADTREE); outputObject.setT(inputObject.getT()); outputObject.addParent(inputObject); inputObject.addChild(outputObject); // Process slice-by-slice for (int z = 0; z < inputObject.getNSlices(); z++) { Roi roi = inputObject.getRoi(z); if (roi == null) continue; try { outputObject.addPointsFromPolygon(roi.getConvexHull(), z); } catch (PointOutOfRangeException e) { } } return outputObject; } public FitConvexHull2D(Modules modules) { super("Fit convex hull 2D", modules); } @Override public String getVersionNumber() { return "1.0.0"; } @Override public String getDescription() { return "Fit 2D convex hull to a 2D object. If objects are in 3D, a convex hull is fit slice-by-slice.

" + "Uses the ImageJ \"Fit convex hull\" function."; } @Override public Category getCategory() { return Categories.OBJECTS_PROCESS; } @Override protected Status process(Workspace workspace) { // Getting input objects String inputObjectsName = parameters.getValue(INPUT_OBJECTS,workspace); Objs inputObjects = workspace.getObjects(inputObjectsName); // Getting parameters String outputObjectsName = parameters.getValue(OUTPUT_OBJECTS,workspace); // If necessary, creating a new Objs and adding it to the Workspace Objs outputObjects = new Objs(outputObjectsName, inputObjects); workspace.addObjects(outputObjects); int count = 0; int total = inputObjects.size(); for (Obj inputObject : inputObjects.values()) { processObject(inputObject, outputObjects); writeProgressStatus(++count, total, "objects"); } if (showOutput) outputObjects.convertToImageIDColours().show(false); return Status.PASS; } @Override protected void initialiseParameters() { parameters.add(new SeparatorP(INPUT_SEPARATOR, this)); parameters.add(new InputObjectsP(INPUT_OBJECTS, this)); parameters.add(new OutputObjectsP(OUTPUT_OBJECTS, this)); addParameterDescriptions(); } @Override public Parameters updateAndGetParameters() { Parameters returnedParameters = new Parameters(); returnedParameters.add(parameters.getParameter(INPUT_SEPARATOR)); returnedParameters.add(parameters.getParameter(INPUT_OBJECTS)); returnedParameters.add(parameters.getParameter(OUTPUT_OBJECTS)); return returnedParameters; } @Override public ImageMeasurementRefs updateAndGetImageMeasurementRefs() { return null; } @Override public ObjMeasurementRefs updateAndGetObjectMeasurementRefs() { return null; } @Override public ObjMetadataRefs updateAndGetObjectMetadataRefs() { return null; } @Override public MetadataRefs updateAndGetMetadataReferences() { return null; } @Override public ParentChildRefs updateAndGetParentChildRefs() { Workspace workspace = null; ParentChildRefs returnedRelationships = new ParentChildRefs(); String inputObjectsName = parameters.getValue(INPUT_OBJECTS,workspace); String outputObjectsName = parameters.getValue(OUTPUT_OBJECTS,workspace); returnedRelationships.add(parentChildRefs.getOrPut(inputObjectsName, outputObjectsName)); return returnedRelationships; } @Override public PartnerRefs updateAndGetPartnerRefs() { return null; } @Override public boolean verify() { return true; } void addParameterDescriptions() { parameters.get(INPUT_OBJECTS).setDescription( "Input objects to create 2D convex hulls for. Each convex hull will be a child of its respective input object."); parameters.get(OUTPUT_OBJECTS).setDescription( "Output convex hull objects will be stored in the workspace with this name. Each convex hull object will be a child of the input object it was created from."); } }




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