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/*
dsh-bio-tools Command line tools.
Copyright (c) 2013-2024 held jointly by the individual authors.
This library is free software; you can redistribute it and/or modify it
under the terms of the GNU Lesser General Public License as published
by the Free Software Foundation; either version 3 of the License, or (at
your option) any later version.
This library is distributed in the hope that it will be useful, but WITHOUT
ANY WARRANTY; with out even the implied warranty of MERCHANTABILITY or
FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public
License for more details.
You should have received a copy of the GNU Lesser General Public License
along with this library; if not, write to the Free Software Foundation,
Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA.
> http://www.fsf.org/licensing/licenses/lgpl.html
> http://www.opensource.org/licenses/lgpl-license.php
*/
package org.dishevelled.bio.tools;
import static org.dishevelled.compress.Readers.reader;
import static org.dishevelled.compress.Writers.writer;
import java.io.BufferedReader;
import java.io.File;
import java.io.PrintWriter;
import java.nio.file.Path;
import java.util.Collections;
import java.util.List;
import java.util.concurrent.Callable;
import org.apache.commons.csv.CSVFormat;
import org.apache.commons.csv.CSVRecord;
import org.dishevelled.bio.variant.vcf.VcfHeader;
import org.dishevelled.bio.variant.vcf.VcfRecord;
import org.dishevelled.bio.variant.vcf.VcfSample;
import org.dishevelled.bio.variant.vcf.VcfWriter;
import org.dishevelled.commandline.ArgumentList;
import org.dishevelled.commandline.CommandLine;
import org.dishevelled.commandline.CommandLineParseException;
import org.dishevelled.commandline.CommandLineParser;
import org.dishevelled.commandline.Switch;
import org.dishevelled.commandline.Usage;
import org.dishevelled.commandline.argument.FileArgument;
import org.dishevelled.commandline.argument.PathArgument;
/**
* Convert Ensembl variant table to VCF format.
*
* @author Michael Heuer
*/
public class EnsemblVariantTableToVcf implements Callable {
private final Path inputEnsemblVariantTablePath;
private final File outputVcfFile;
private static final String USAGE = "dsh-variant-table-to-vcf [args]";
/**
* Convert Ensembl variant table to VCF format
*
* @deprecated will be removed in version 3.0
* @param inputEnsemblVariantTableFile input Ensembl variant table file, if any
* @param outputVcfFile output VCF file, if any
*/
public EnsemblVariantTableToVcf(final File inputEnsemblVariantTableFile, final File outputVcfFile) {
this(inputEnsemblVariantTableFile == null ? null : inputEnsemblVariantTableFile.toPath(), outputVcfFile);
}
/**
* Convert Ensembl variant table to VCF format
*
* @since 2.1
* @param inputEnsemblVariantTablePath input Ensembl variant table path, if any
* @param outputVcfFile output VCF file, if any
*/
public EnsemblVariantTableToVcf(final Path inputEnsemblVariantTablePath, final File outputVcfFile) {
this.inputEnsemblVariantTablePath = inputEnsemblVariantTablePath;
this.outputVcfFile = outputVcfFile;
}
@Override
public Integer call() throws Exception {
BufferedReader reader = null;
PrintWriter writer = null;
try {
reader = reader(inputEnsemblVariantTablePath);
writer = writer(outputVcfFile);
VcfHeader header = VcfHeader.builder()
.withFileFormat("VCFv4.2")
.withMeta("##fileformat=VCFv4.2")
.build();
VcfWriter.writeHeader(header, writer);
List samples = Collections.emptyList();
VcfWriter.writeColumnHeader(samples, writer);
VcfRecord.Builder builder = VcfRecord.builder()
.withFilter("PASS")
.withFormat("");
for (CSVRecord record : CSVFormat.DEFAULT.withHeader().parse(reader)) {
String variantId = record.get("Variant ID");
String[] location = record.get("Location").split(":");
String[] alleles = record.get("Alleles").replace("-", ".").split("/");
if (alleles.length != 2) {
// log warning, found e.g. COSMIC_MUTATION, HGMD_MUTATION, or CCC/-/CC, G/A/C/T
continue;
}
VcfRecord vcfRecord = builder.withChrom(location[0])
.withPos(Long.parseLong(location[1]))
.withRef(alleles[0])
.withAlt(alleles[1])
.withId(variantId)
.build();
VcfWriter.writeRecord(samples, vcfRecord, writer);
}
return 0;
}
finally {
try {
reader.close();
}
catch (Exception e) {
// ignore
}
try {
writer.close();
}
catch (Exception e) {
// ignore
}
}
}
/**
* Main.
*
* @param args command line args
*/
public static void main(final String[] args) {
Switch about = new Switch("a", "about", "display about message");
Switch help = new Switch("h", "help", "display help message");
PathArgument inputEnsemblVariantTablePath = new PathArgument("i", "input-variant-table-path", "input Ensembl variant table path, default stdin", false);
FileArgument outputVcfFile = new FileArgument("o", "output-vcf-file", "output VCF file, default stdout", false);
ArgumentList arguments = new ArgumentList(about, help, inputEnsemblVariantTablePath, outputVcfFile);
CommandLine commandLine = new CommandLine(args);
EnsemblVariantTableToVcf ensemblVariantTableToVcf = null;
try {
CommandLineParser.parse(commandLine, arguments);
if (about.wasFound()) {
About.about(System.out);
System.exit(0);
}
if (help.wasFound()) {
Usage.usage(USAGE, null, commandLine, arguments, System.out);
System.exit(0);
}
ensemblVariantTableToVcf = new EnsemblVariantTableToVcf(inputEnsemblVariantTablePath.getValue(), outputVcfFile.getValue());
}
catch (CommandLineParseException e) {
if (about.wasFound()) {
About.about(System.out);
System.exit(0);
}
if (help.wasFound()) {
Usage.usage(USAGE, null, commandLine, arguments, System.out);
System.exit(0);
}
Usage.usage(USAGE, e, commandLine, arguments, System.err);
System.exit(-1);
}
catch (IllegalArgumentException e) {
Usage.usage(USAGE, e, commandLine, arguments, System.err);
System.exit(-1);
}
try {
System.exit(ensemblVariantTableToVcf.call());
}
catch (Exception e) {
e.printStackTrace();
System.exit(1);
}
}
}
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