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/*
dsh-bio-tools Command line tools.
Copyright (c) 2013-2024 held jointly by the individual authors.
This library is free software; you can redistribute it and/or modify it
under the terms of the GNU Lesser General Public License as published
by the Free Software Foundation; either version 3 of the License, or (at
your option) any later version.
This library is distributed in the hope that it will be useful, but WITHOUT
ANY WARRANTY; with out even the implied warranty of MERCHANTABILITY or
FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public
License for more details.
You should have received a copy of the GNU Lesser General Public License
along with this library; if not, write to the Free Software Foundation,
Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA.
> http://www.fsf.org/licensing/licenses/lgpl.html
> http://www.opensource.org/licenses/lgpl-license.php
*/
package org.dishevelled.bio.tools;
import static org.dishevelled.compress.Readers.reader;
import static org.dishevelled.compress.Writers.writer;
import java.io.BufferedReader;
import java.io.File;
import java.io.PrintWriter;
import java.nio.file.Path;
import java.util.concurrent.Callable;
import com.google.common.base.Joiner;
import org.dishevelled.bio.assembly.gfa1.Gfa1Adapter;
import org.dishevelled.bio.assembly.gfa1.Gfa1Reader;
import org.dishevelled.bio.assembly.gfa1.Segment;
import org.dishevelled.commandline.ArgumentList;
import org.dishevelled.commandline.CommandLine;
import org.dishevelled.commandline.CommandLineParseException;
import org.dishevelled.commandline.CommandLineParser;
import org.dishevelled.commandline.Switch;
import org.dishevelled.commandline.Usage;
import org.dishevelled.commandline.argument.FileArgument;
import org.dishevelled.commandline.argument.IntegerArgument;
import org.dishevelled.commandline.argument.PathArgument;
/**
* Export assembly segment sequences in GFA 1.0 format to FASTA format.
*
* @since 2.1
* @author Michael Heuer
*/
public final class ExportSegments implements Callable {
private final Path inputGfa1Path;
private final File outputFastaFile;
private final int lineWidth;
static final int DEFAULT_LINE_WIDTH = 70;
private static final String USAGE = "dsh-export-segments [args]";
/**
* Export assembly segment sequences in GFA 1.0 format to FASTA format.
*
* @deprecated will be removed in version 3.0
* @param inputGfa1File input GFA 1.0 file, if any
* @param outputFastaFile output FASTA file, if any
* @param lineWidth line width
*/
public ExportSegments(final File inputGfa1File, final File outputFastaFile, final int lineWidth) {
this(inputGfa1File == null ? null : inputGfa1File.toPath(), outputFastaFile, lineWidth);
}
/**
* Export assembly segment sequences in GFA 1.0 format to FASTA format.
*
* @since 2.1
* @param inputGfa1Path input GFA 1.0 path, if any
* @param outputFastaFile output FASTA file, if any
* @param lineWidth line width
*/
public ExportSegments(final Path inputGfa1Path, final File outputFastaFile, final int lineWidth) {
this.inputGfa1Path = inputGfa1Path;
this.outputFastaFile = outputFastaFile;
this.lineWidth = lineWidth;
}
@Override
public Integer call() throws Exception {
BufferedReader reader = null;
PrintWriter writer = null;
try {
reader = reader(inputGfa1Path);
writer = writer(outputFastaFile);
final PrintWriter w = writer;
Gfa1Reader.stream(reader, new Gfa1Adapter() {
@Override
public boolean segment(final Segment segment) {
if (segment.hasSequence()) {
w.print(">");
w.println(describeSegment(segment));
String sequence = segment.getSequence();
for (int i = 0, length = sequence.length(); i <= length; i += lineWidth) {
w.println(sequence.substring(i, Math.min(i + lineWidth, length)));
}
}
return true;
}
});
return 0;
}
finally {
try {
reader.close();
}
catch (Exception e) {
// ignore
}
try {
writer.close();
}
catch (Exception e) {
// ignore
}
}
}
static String describeSegment(final Segment segment) {
StringBuilder sb = new StringBuilder();
sb.append(segment.getName());
if (!segment.getAnnotations().isEmpty()) {
sb.append(" ");
Joiner joiner = Joiner.on("\t");
joiner.appendTo(sb, segment.getAnnotations().values());
}
return sb.toString();
}
/**
* Main.
*
* @param args command line args
*/
public static void main(final String[] args) {
Switch about = new Switch("a", "about", "display about message");
Switch help = new Switch("h", "help", "display help message");
PathArgument inputGfa1Path = new PathArgument("i", "input-gfa1-path", "input GFA 1.0 path, default stdin", false);
FileArgument outputFastaFile = new FileArgument("o", "output-fasta-file", "output FASTA file, default stdout", false);
IntegerArgument lineWidth = new IntegerArgument("w", "line-width", "line width, default " + DEFAULT_LINE_WIDTH, false);
ArgumentList arguments = new ArgumentList(about, help, inputGfa1Path, outputFastaFile, lineWidth);
CommandLine commandLine = new CommandLine(args);
ExportSegments exportSegments = null;
try
{
CommandLineParser.parse(commandLine, arguments);
if (about.wasFound()) {
About.about(System.out);
System.exit(0);
}
if (help.wasFound()) {
Usage.usage(USAGE, null, commandLine, arguments, System.out);
System.exit(0);
}
exportSegments = new ExportSegments(inputGfa1Path.getValue(), outputFastaFile.getValue(), lineWidth.getValue(DEFAULT_LINE_WIDTH));
}
catch (CommandLineParseException e) {
Usage.usage(USAGE, e, commandLine, arguments, System.err);
System.exit(-1);
}
try {
System.exit(exportSegments.call());
}
catch (Exception e) {
e.printStackTrace();
System.exit(1);
}
}
}
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