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/*
dsh-bio-tools Command line tools.
Copyright (c) 2013-2024 held jointly by the individual authors.
This library is free software; you can redistribute it and/or modify it
under the terms of the GNU Lesser General Public License as published
by the Free Software Foundation; either version 3 of the License, or (at
your option) any later version.
This library is distributed in the hope that it will be useful, but WITHOUT
ANY WARRANTY; with out even the implied warranty of MERCHANTABILITY or
FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public
License for more details.
You should have received a copy of the GNU Lesser General Public License
along with this library; if not, write to the Free Software Foundation,
Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA.
> http://www.fsf.org/licensing/licenses/lgpl.html
> http://www.opensource.org/licenses/lgpl-license.php
*/
package org.dishevelled.bio.tools;
import static org.dishevelled.compress.Readers.reader;
import static org.dishevelled.compress.Writers.writer;
import java.io.BufferedReader;
import java.io.File;
import java.io.IOException;
import java.io.PrintWriter;
import java.nio.file.Path;
import java.util.concurrent.Callable;
import java.util.regex.Matcher;
import java.util.regex.Pattern;
import java.util.regex.PatternSyntaxException;
import org.biojava.bio.seq.Sequence;
import org.biojava.bio.seq.SequenceIterator;
import org.biojava.bio.seq.io.SeqIOTools;
import org.dishevelled.commandline.ArgumentList;
import org.dishevelled.commandline.CommandLine;
import org.dishevelled.commandline.CommandLineParseException;
import org.dishevelled.commandline.CommandLineParser;
import org.dishevelled.commandline.Switch;
import org.dishevelled.commandline.Usage;
import org.dishevelled.commandline.argument.FileArgument;
import org.dishevelled.commandline.argument.IntegerArgument;
import org.dishevelled.commandline.argument.StringArgument;
import org.dishevelled.commandline.argument.PathArgument;
/**
* Extract matching DNA or protein sequences in FASTA format.
*
* @author Michael Heuer
*/
@SuppressWarnings("deprecation")
public final class ExtractFasta implements Callable {
private final Path inputFastaPath;
private final File outputFastaFile;
private final String alphabet;
private final String name;
private final Pattern pattern;
private final int lineWidth;
static final String DEFAULT_ALPHABET = "dna";
static final int DEFAULT_LINE_WIDTH = 70;
static final String DESCRIPTION_LINE = "description_line";
private static final String USAGE = "dsh-extract-fasta [args]";
/**
* Extract matching DNA or protein sequences in FASTA format.
*
* @deprecated will be removed in version 3.0
* @param inputFastaFile input FASTA file, if any
* @param outputFastaFile output FASTA file, if any
* @param name exact sequence name to match, if any
* @param description FASTA description line regex pattern to match, if any
* @param lineWidth line width
*/
public ExtractFasta(final File inputFastaFile,
final File outputFastaFile,
final String name,
final String description,
final int lineWidth) {
this(inputFastaFile == null ? null : inputFastaFile.toPath(),
outputFastaFile,
name,
description,
DEFAULT_ALPHABET,
lineWidth);
}
/**
* Extract matching DNA or protein sequences in FASTA format.
*
* @since 2.1
* @param inputFastaPath input FASTA path, if any
* @param outputFastaFile output FASTA file, if any
* @param name exact sequence name to match, if any
* @param description FASTA description line regex pattern to match, if any
* @param lineWidth line width
*/
public ExtractFasta(final Path inputFastaPath,
final File outputFastaFile,
final String name,
final String description,
final int lineWidth) {
this(inputFastaPath, outputFastaFile, name, description, DEFAULT_ALPHABET, lineWidth);
}
/**
* Extract matching DNA or protein sequences in FASTA format.
*
* @since 2.0
* @deprecated will be removed in version 3.0
* @param inputFastaFile input FASTA file, if any
* @param outputFastaFile output FASTA file, if any
* @param name exact sequence name to match, if any
* @param description FASTA description line regex pattern to match, if any
* @param alphabet input FASTA file alphabet { dna, protein }, if any
* @param lineWidth line width
*/
public ExtractFasta(final File inputFastaFile,
final File outputFastaFile,
final String name,
final String description,
final String alphabet,
final int lineWidth) {
this(inputFastaFile == null ? null : inputFastaFile.toPath(),
outputFastaFile,
name,
description,
alphabet,
lineWidth);
}
/**
* Extract matching DNA or protein sequences in FASTA format.
*
* @since 2.1
* @param inputFastaPath input FASTA path, if any
* @param outputFastaFile output FASTA file, if any
* @param name exact sequence name to match, if any
* @param description FASTA description line regex pattern to match, if any
* @param alphabet input FASTA file alphabet { dna, protein }, if any
* @param lineWidth line width
*/
public ExtractFasta(final Path inputFastaPath,
final File outputFastaFile,
final String name,
final String description,
final String alphabet,
final int lineWidth) {
this.inputFastaPath = inputFastaPath;
this.outputFastaFile = outputFastaFile;
this.name = name;
try {
this.pattern = (description == null) ? null : Pattern.compile(description);
}
catch (PatternSyntaxException e) {
throw new IllegalArgumentException("illegal regex pattern, caught " + e.getMessage());
}
this.alphabet = alphabet;
this.lineWidth = lineWidth;
}
@Override
public Integer call() throws Exception {
BufferedReader reader = null;
PrintWriter writer = null;
try {
reader = reader(inputFastaPath);
writer = writer(outputFastaFile);
for (SequenceIterator sequences = isProteinAlphabet() ? SeqIOTools.readFastaProtein(reader) : SeqIOTools.readFastaDNA(reader); sequences.hasNext(); ) {
Sequence sequence = sequences.nextSequence();
if (sequence.getName().equals(name)) {
writeSequence(sequence, lineWidth, writer);
}
else if (pattern != null) {
Matcher matcher = pattern.matcher(describeSequence(sequence));
if (matcher.matches()) {
writeSequence(sequence, lineWidth, writer);
}
}
}
return 0;
}
finally {
try {
reader.close();
}
catch (Exception e) {
// ignore
}
try {
writer.close();
}
catch (Exception e) {
// ignore
}
}
}
boolean isProteinAlphabet() {
return alphabet != null && (alphabet.equalsIgnoreCase("protein") || alphabet.equalsIgnoreCase("aa"));
}
// copied with mods from biojava-legacy FastaFormat, as it uses PrintStream not PrintWriter
static String describeSequence(final Sequence sequence) {
return sequence.getAnnotation().containsProperty(DESCRIPTION_LINE) ?
(String) sequence.getAnnotation().getProperty(DESCRIPTION_LINE) : (String) sequence.getName();
}
static void writeSequence(final Sequence sequence, final int lineWidth, final PrintWriter writer) throws IOException {
writer.print(">");
writer.println(describeSequence(sequence));
for (int i = 1, length = sequence.length(); i <= length; i += lineWidth) {
writer.println(sequence.subStr(i, Math.min(i + lineWidth - 1, length)));
}
}
/**
* Main.
*
* @param args command line args
*/
public static void main(final String[] args) {
Switch about = new Switch("a", "about", "display about message");
Switch help = new Switch("h", "help", "display help message");
PathArgument inputFastaPath = new PathArgument("i", "input-fasta-path", "input FASTA path, default stdin", false);
FileArgument outputFastaFile = new FileArgument("o", "output-fasta-file", "output FASTA file, default stdout", false);
StringArgument name = new StringArgument("n", "name", "exact sequence name to match", false);
StringArgument description = new StringArgument("d", "description", "FASTA description line regex pattern to match", false);
StringArgument alphabet = new StringArgument("e", "alphabet", "input FASTA alphabet { dna, protein }, default dna", false);
IntegerArgument lineWidth = new IntegerArgument("w", "line-width", "line width, default " + DEFAULT_LINE_WIDTH, false);
ArgumentList arguments = new ArgumentList(about, help, inputFastaPath, outputFastaFile, name, description, alphabet, lineWidth);
CommandLine commandLine = new CommandLine(args);
ExtractFasta extractFasta = null;
try
{
CommandLineParser.parse(commandLine, arguments);
if (about.wasFound()) {
About.about(System.out);
System.exit(0);
}
if (help.wasFound()) {
Usage.usage(USAGE, null, commandLine, arguments, System.out);
System.exit(0);
}
extractFasta = new ExtractFasta(inputFastaPath.getValue(), outputFastaFile.getValue(), name.getValue(), description.getValue(), alphabet.getValue(DEFAULT_ALPHABET), lineWidth.getValue(DEFAULT_LINE_WIDTH));
}
catch (CommandLineParseException e) {
Usage.usage(USAGE, e, commandLine, arguments, System.err);
System.exit(-1);
}
try {
System.exit(extractFasta.call());
}
catch (Exception e) {
e.printStackTrace();
System.exit(1);
}
}
}
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