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Next generation sequencing (NGS/HTS) reads.
/*
ngs-reads Next generation sequencing (NGS/HTS) reads.
Copyright (c) 2014 National Marrow Donor Program (NMDP)
This library is free software; you can redistribute it and/or modify it
under the terms of the GNU Lesser General Public License as published
by the Free Software Foundation; either version 3 of the License, or (at
your option) any later version.
This library is distributed in the hope that it will be useful, but WITHOUT
ANY WARRANTY; with out even the implied warranty of MERCHANTABILITY or
FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public
License for more details.
You should have received a copy of the GNU Lesser General Public License
along with this library; if not, write to the Free Software Foundation,
Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA.
> http://www.gnu.org/licenses/lgpl.html
*/
package org.nmdp.ngs.reads.paired;
import static com.google.common.base.Preconditions.checkNotNull;
import java.io.IOException;
import java.io.Reader;
import java.util.Collections;
import java.util.List;
import java.util.concurrent.Callable;
import java.util.concurrent.ConcurrentMap;
import java.util.concurrent.ConcurrentHashMap;
import java.util.concurrent.ExecutionException;
import java.util.concurrent.Executors;
import java.util.concurrent.ExecutorService;
import java.util.concurrent.Future;
import java.util.regex.Matcher;
import java.util.regex.Pattern;
import com.google.common.collect.ImmutableList;
import com.google.common.collect.Ordering;
import com.google.common.collect.Lists;
import org.biojava.bio.program.fastq.Fastq;
import org.biojava.bio.program.fastq.SangerFastqReader;
import org.biojava.bio.program.fastq.StreamListener;
/**
* Paired end FASTQ reads reader.
*/
public final class PairedEndFastqReader {
/** Pattern for the left or first read of a paired end read, relies on convention of " 1" in the description line. */
static final Pattern LEFT = Pattern.compile("^.* 1.*$");
/** Pattern for the right or second read of a paired end read, relies on convention of " 2" in the description line. */
static final Pattern RIGHT = Pattern.compile("^.* 2.*$");
/** Pattern for capturing the prefix of a paired end read name, relies on convention of " 1" or " 2" in the description line. */
static final Pattern PREFIX = Pattern.compile("^(.+) [12].*$");
/**
* Private no-arg constructor.
*/
private PairedEndFastqReader() {
// empty
}
/**
* Read the specified paired end reads. The paired end reads are read fully into RAM before processing.
*
* @param firstReader first reader, must not be null
* @param secondReader second reader, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
* @deprecated by {@link #readPaired(Readable,Readable,PairedEndListener)}, will be removed in version 2.0
*/
public static void readPaired(final Reader firstReader,
final Reader secondReader,
final PairedEndListener listener) throws IOException {
readPaired((Readable) firstReader, (Readable) secondReader, listener);
}
/**
* Read the specified paired end reads. The paired end reads are read fully into RAM before processing.
*
* @param firstReadable first readable, must not be null
* @param secondReadable second readable, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
*/
public static void readPaired(final Readable firstReadable,
final Readable secondReadable,
final PairedEndListener listener) throws IOException {
checkNotNull(firstReadable);
checkNotNull(secondReadable);
checkNotNull(listener);
// read both FASTQ files into RAM (ick)
final List reads = Lists.newArrayList();
SangerFastqReader fastqReader = new SangerFastqReader();
fastqReader.stream(firstReadable, new StreamListener() {
@Override
public void fastq(final Fastq fastq) {
reads.add(fastq);
}
});
fastqReader.stream(secondReadable, new StreamListener() {
@Override
public void fastq(final Fastq fastq) {
reads.add(fastq);
}
});
// .. and sort by description
Collections.sort(reads, new Ordering() {
@Override
public int compare(final Fastq left, final Fastq right) {
return left.getDescription().compareTo(right.getDescription());
}
});
for (int i = 0, size = reads.size(); i < size; ) {
Fastq left = reads.get(i);
if ((i + 1) == size) {
listener.unpaired(left);
break;
}
Fastq right = reads.get(i + 1);
if (isLeft(left)) {
if (isRight(right)) {
listener.paired(left, right);
i += 2;
}
else {
listener.unpaired(right);
i++;
}
}
else {
listener.unpaired(left);
i++;
}
}
}
/**
* Stream the specified paired end reads. RAM usage is minimal if the paired end reads are sorted.
*
* @param firstReader first reader, must not be null
* @param secondReader second reader, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
* @deprecated by {@link #streamPaired(Readable,Readable,PairedEndListener)}, will be removed in version 2.0
*/
public static void streamPaired(final Reader firstReader,
final Reader secondReader,
final PairedEndListener listener) throws IOException {
streamPaired((Readable) firstReader, (Readable) secondReader, listener);
}
/**
* Stream the specified paired end reads. RAM usage is minimal if the paired end reads are sorted.
*
* @param firstReadable first readable, must not be null
* @param secondReadable second readable, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
*/
public static void streamPaired(final Readable firstReadable,
final Readable secondReadable,
final PairedEndListener listener) throws IOException {
checkNotNull(firstReadable);
checkNotNull(secondReadable);
checkNotNull(listener);
final ConcurrentMap keyedByPrefix = new ConcurrentHashMap<>();
final StreamListener streamListener = new StreamListener() {
@Override
public void fastq(final Fastq fastq) {
String prefix = prefix(fastq);
Fastq other = keyedByPrefix.putIfAbsent(prefix, fastq);
if ((other != null) && !fastq.equals(other)) {
if (isLeft(other) && isRight(fastq)) {
listener.paired(other, fastq);
}
else if (isRight(other) && isLeft(fastq)) {
listener.paired(fastq, other);
}
else {
throw new PairedEndFastqReaderException("fastq " + fastq + " other " + other);
}
keyedByPrefix.remove(prefix);
}
}
};
try {
ExecutorService executor = Executors.newFixedThreadPool(2);
Callable task1 = new Callable() {
@Override
public Void call() throws IOException {
new SangerFastqReader().stream(firstReadable, streamListener);
return null;
}
};
Callable task2 = new Callable() {
@Override
public Void call() throws IOException {
new SangerFastqReader().stream(secondReadable, streamListener);
return null;
}
};
for (Future future : executor.invokeAll(ImmutableList.of(task1, task2))) {
future.get();
}
executor.shutdown();
}
catch (ExecutionException e) {
throw new IOException(e.getCause());
}
catch (InterruptedException e) {
// ignore
}
catch (PairedEndFastqReaderException e) {
throw new IOException("could not read paired end FASTQ reads", e);
}
for (Fastq unpaired : keyedByPrefix.values()) {
listener.unpaired(unpaired);
}
}
/**
* Stream the specified interleaved paired end reads. Per the interleaved format, all reads must be sorted and paired.
*
* @param reader reader, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
* @deprecated by {@link #streamInterleaved(Readable,PairedEndListener)}, will be removed in version 2.0
*/
public static void streamInterleaved(final Reader reader, final PairedEndListener listener) throws IOException {
streamInterleaved((Readable) reader, listener);
}
/**
* Stream the specified interleaved paired end reads. Per the interleaved format, all reads must be sorted and paired.
*
* @param readable readable, must not be null
* @param listener paired end listener, must not be null
* @throws IOException if an I/O error occurs
*/
public static void streamInterleaved(final Readable readable, final PairedEndListener listener) throws IOException {
checkNotNull(readable);
checkNotNull(listener);
StreamListener streamListener = new StreamListener() {
private Fastq left;
@Override
public void fastq(final Fastq fastq) {
if (isLeft(fastq) && (left == null)) {
left = fastq;
}
else if (isRight(fastq) && (left != null)) {
Fastq right = fastq;
listener.paired(left, right);
left = null;
}
else {
throw new PairedEndFastqReaderException("invalid interleaved FASTQ format, left=" + (left == null ? "null" : left.getDescription()) + " right=" + (fastq == null ? "null" : fastq.getDescription()));
}
}
};
try {
new SangerFastqReader().stream(readable, streamListener);
}
catch (PairedEndFastqReaderException e) {
throw new IOException("could not stream interleaved paired end FASTQ reads", e);
}
}
/**
* Return true if the specified fastq is the left or first read of a paired end read.
*
* @param fastq fastq, must not be null
* @return true if the specified fastq is the left or first read of a paired end read
*/
static boolean isLeft(final Fastq fastq) {
checkNotNull(fastq);
return LEFT.matcher(fastq.getDescription()).matches();
}
/**
* Return true if the specified fastq is the right or second read of a paired end read.
*
* @param fastq fastq, must not be null
* @return true if the specified fastq is the right or second8 read of a paired end read
*/
static boolean isRight(final Fastq fastq) {
checkNotNull(fastq);
return RIGHT.matcher(fastq.getDescription()).matches();
}
/**
* Return the prefix of the paired end read name of the specified fastq.
*
* @param fastq fastq, must not be null
* @return the prefix of the paired end read name of the specified fastq
*/
static String prefix(final Fastq fastq) {
checkNotNull(fastq);
Matcher m = PREFIX.matcher(fastq.getDescription());
if (!m.matches()) {
throw new PairedEndFastqReaderException("could not parse prefix from description " + fastq.getDescription());
}
return m.group(1);
}
}