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/*
* Copyright 2015-2020 OpenCB
*
* Licensed under the Apache License, Version 2.0 (the "License");
* you may not use this file except in compliance with the License.
* You may obtain a copy of the License at
*
* http://www.apache.org/licenses/LICENSE-2.0
*
* Unless required by applicable law or agreed to in writing, software
* distributed under the License is distributed on an "AS IS" BASIS,
* WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
* See the License for the specific language governing permissions and
* limitations under the License.
*/
package org.opencb.cellbase.lib.managers;
import org.opencb.biodata.models.core.*;
import org.opencb.biodata.models.variant.Variant;
import org.opencb.biodata.models.variant.VariantBuilder;
import org.opencb.biodata.models.variant.avro.SampleEntry;
import org.opencb.biodata.models.variant.avro.Score;
import org.opencb.biodata.models.variant.avro.VariantAnnotation;
import org.opencb.biodata.models.variant.avro.VariantType;
import org.opencb.cellbase.core.ParamConstants;
import org.opencb.cellbase.core.api.SnpQuery;
import org.opencb.cellbase.core.api.VariantQuery;
import org.opencb.cellbase.core.api.key.ApiKeyLicensedDataUtils;
import org.opencb.cellbase.core.api.query.CellBaseQueryOptions;
import org.opencb.cellbase.core.api.query.QueryException;
import org.opencb.cellbase.core.config.CellBaseConfiguration;
import org.opencb.cellbase.core.exception.CellBaseException;
import org.opencb.cellbase.core.models.DataRelease;
import org.opencb.cellbase.core.result.CellBaseDataResult;
import org.opencb.cellbase.core.variant.AnnotationBasedPhasedQueryManager;
import org.opencb.cellbase.lib.impl.core.CellBaseCoreDBAdaptor;
import org.opencb.cellbase.lib.impl.core.SnpMongoDBAdaptor;
import org.opencb.cellbase.lib.impl.core.SpliceScoreMongoDBAdaptor;
import org.opencb.cellbase.lib.impl.core.VariantMongoDBAdaptor;
import org.opencb.cellbase.lib.variant.VariantAnnotationUtils;
import org.opencb.cellbase.lib.variant.annotation.CellBaseNormalizerSequenceAdaptor;
import org.opencb.cellbase.lib.variant.annotation.VariantAnnotationCalculator;
import org.opencb.cellbase.lib.variant.hgvs.HgvsCalculator;
import org.opencb.commons.datastore.core.Query;
import org.opencb.commons.datastore.core.QueryOptions;
import java.util.*;
import java.util.concurrent.ExecutionException;
import java.util.stream.Collectors;
public class VariantManager extends AbstractManager implements AggregationApi {
private static final String PHASE_DATA_URL_SEPARATOR = "\\+";
private static final String VARIANT_STRING_FORMAT = "(chr)"
+ ":[(cipos_left)<](start)[<(cipos_right)]" + "[-[(ciend_left)<](end)[<(ciend_right)]]"
+ "[:(ref)]"
+ ":[(alt)|(left_ins_seq)...(right_ins_seq)]";
private VariantMongoDBAdaptor variantDBAdaptor;
private SpliceScoreMongoDBAdaptor spliceDBAdaptor;
private SnpMongoDBAdaptor snpDBAdaptor;
private CellBaseManagerFactory cellbaseManagerFactory;
private GenomeManager genomeManager;
public VariantManager(String species, CellBaseConfiguration configuration) throws CellBaseException {
this(species, null, configuration);
}
public VariantManager(String species, String assembly, CellBaseConfiguration configuration)
throws CellBaseException {
super(species, assembly, configuration);
this.init();
}
private void init() throws CellBaseException {
variantDBAdaptor = dbAdaptorFactory.getVariationDBAdaptor();
spliceDBAdaptor = dbAdaptorFactory.getSpliceScoreDBAdaptor();
snpDBAdaptor = dbAdaptorFactory.getSnpDBAdaptor();
cellbaseManagerFactory = new CellBaseManagerFactory(configuration);
genomeManager = cellbaseManagerFactory.getGenomeManager(species, assembly);
}
@Override
public CellBaseCoreDBAdaptor getDBAdaptor() {
return variantDBAdaptor;
}
public CellBaseDataResult get(Query query, QueryOptions queryOptions, int dataRelease) throws CellBaseException {
return variantDBAdaptor.nativeGet(query, queryOptions, dataRelease);
}
public List> getHgvsByVariant(String variants, DataRelease dataRelease)
throws CellBaseException, QueryException, IllegalAccessException {
List variantList = parseVariants(variants);
HgvsCalculator hgvsCalculator = new HgvsCalculator(genomeManager, dataRelease.getRelease());
List> results = new ArrayList<>();
VariantAnnotationCalculator variantAnnotationCalculator = new VariantAnnotationCalculator(species, assembly,
dataRelease, "", cellbaseManagerFactory);
List batchGeneList = variantAnnotationCalculator.getBatchGeneList(variantList);
for (Variant variant : variantList) {
List variantGeneList = variantAnnotationCalculator.getAffectedGenes(batchGeneList, variant);
List hgvsStrings = hgvsCalculator.run(variant, variantGeneList, false);
results.add(new CellBaseDataResult<>(variant.getId(), 0, new ArrayList<>(), hgvsStrings.size(), hgvsStrings, -1));
}
return results;
}
/**
* Normalises a list of variants.
*
* @param variants list of variant strings
* @param decompose boolean to set the decompose MNV behaviour
* @param leftAlign boolean to set the left alignment behaviour
* @param dataRelease data release
* @return list of normalised variants
* @throws CellBaseException if the species is incorrect
*/
public CellBaseDataResult getNormalizationByVariant(String variants, boolean decompose, boolean leftAlign,
DataRelease dataRelease) throws CellBaseException {
List variantList = parseVariants(variants);
VariantAnnotationCalculator variantAnnotationCalculator = new VariantAnnotationCalculator(species, assembly,
dataRelease, "", cellbaseManagerFactory);
// Set decompose MNV behaviour
variantAnnotationCalculator.getNormalizer().getConfig().setDecomposeMNVs(decompose);
// Set left alignment behaviour
if (leftAlign) {
variantAnnotationCalculator.getNormalizer().getConfig().enableLeftAlign(new CellBaseNormalizerSequenceAdaptor(genomeManager,
dataRelease.getRelease()));
} else {
variantAnnotationCalculator.getNormalizer().getConfig().disableLeftAlign();
}
List normalisedVariants = variantAnnotationCalculator.normalizer(variantList);
return new CellBaseDataResult<>(variants, 0, new ArrayList<>(), normalisedVariants.size(), normalisedVariants, -1);
}
public List> getAnnotationByVariant(QueryOptions queryOptions,
String variants,
Boolean normalize,
Boolean decompose,
Boolean leftAlign,
Boolean ignorePhase,
@Deprecated Boolean phased,
Boolean imprecise,
Integer svExtraPadding,
Integer cnvExtraPadding,
Boolean checkAminoAcidChange,
String consequenceTypeSource,
DataRelease dataRelease,
String apiKey)
throws ExecutionException, InterruptedException, CellBaseException, QueryException, IllegalAccessException {
List variantList = parseVariants(variants);
logger.debug("queryOptions: " + queryOptions);
// If ignorePhase (new parameter) is present, then overrides presence of "phased"
if (ignorePhase != null) {
queryOptions.put("ignorePhase", ignorePhase);
// If the new parameter (ignorePhase) is not present but old one ("phased") is, then follow old one - probably
// someone who has not moved to the new parameter yet
} else if (phased != null) {
queryOptions.put("ignorePhase", !phased);
// Default behavior is to perform phased annotation
} else {
queryOptions.put("ignorePhase", false);
}
if (normalize != null) {
queryOptions.put("normalize", normalize);
}
if (decompose != null) {
queryOptions.put("decompose", decompose);
}
if (leftAlign != null) {
queryOptions.put("leftAlign", leftAlign);
}
if (imprecise != null) {
queryOptions.put("imprecise", imprecise);
}
if (svExtraPadding != null) {
queryOptions.put("svExtraPadding", svExtraPadding);
}
if (cnvExtraPadding != null) {
queryOptions.put("cnvExtraPadding", cnvExtraPadding);
}
if (checkAminoAcidChange != null) {
queryOptions.put("checkAminoAcidChange", checkAminoAcidChange);
}
if (consequenceTypeSource != null) {
queryOptions.put("consequenceTypeSource", consequenceTypeSource);
}
VariantAnnotationCalculator variantAnnotationCalculator = new VariantAnnotationCalculator(species, assembly,
dataRelease, apiKey, cellbaseManagerFactory);
List> queryResults = variantAnnotationCalculator.getAnnotationByVariantList(variantList,
queryOptions);
return queryResults;
}
private List parseVariants(String variantsString) {
List variants = null;
if (variantsString != null && !variantsString.isEmpty()) {
String[] variantItems = variantsString.split(",");
variants = new ArrayList<>(variantItems.length);
for (String variantString: variantItems) {
variants.add(parseVariant(variantString));
}
}
return variants;
}
private Variant parseVariant(String variantString) {
String[] variantStringPartArray = variantString.split(PHASE_DATA_URL_SEPARATOR);
VariantBuilder variantBuilder;
if (variantStringPartArray.length > 0) {
variantBuilder = new VariantBuilder(variantStringPartArray[0]);
// Either 1 or 3 parts expected variant+GT+PS
if (variantStringPartArray.length == 3) {
List formatList = new ArrayList<>(2);
// If phase set tag is not provided not phase data is added at all to the Variant object
if (!variantStringPartArray[2].isEmpty()) {
formatList.add(AnnotationBasedPhasedQueryManager.PHASE_SET_TAG);
List sampleData = new ArrayList<>(2);
sampleData.add(variantStringPartArray[2]);
// Genotype field might be empty - just PS would be added to Variant object in that case
if (!variantStringPartArray[1].isEmpty()) {
formatList.add(AnnotationBasedPhasedQueryManager.GENOTYPE_TAG);
sampleData.add(variantStringPartArray[1]);
}
variantBuilder.setSampleDataKeys(formatList);
SampleEntry sampleEntry = new SampleEntry();
sampleEntry.setData(sampleData);
variantBuilder.setSamples(Collections.singletonList(sampleEntry));
}
} else if (variantStringPartArray.length > 3) {
throw new IllegalArgumentException("Malformed variant string " + variantString + ". "
+ "variantString+GT+PS expected, where variantString needs 3 or 4 fields separated by ':'. "
+ "Format: \"" + VARIANT_STRING_FORMAT + "\"");
}
} else {
throw new IllegalArgumentException("Malformed variant string " + variantString + ". "
+ "variantString+GT+PS expected, where variantString needs 3 or 4 fields separated by ':'. "
+ "Format: \"" + VARIANT_STRING_FORMAT + "\"");
}
return variantBuilder.build();
}
public CellBaseDataResult getConsequenceTypes() {
List consequenceTypes = VariantAnnotationUtils.SO_SEVERITY.keySet().stream()
.sorted()
.collect(Collectors.toList());
CellBaseDataResult queryResult = new CellBaseDataResult<>("consequence_types");
queryResult.setNumResults(consequenceTypes.size());
queryResult.setResults(consequenceTypes);
return queryResult;
}
public boolean validateRegionInput(String regions) {
List regionList = Region.parseRegions(regions);
// check for regions bigger than 10Mb
if (regionList != null) {
for (Region r : regionList) {
if ((r.getEnd() - r.getStart()) > 10000000) {
return false;
}
}
}
return true;
}
@Deprecated
public List getByRegion(Query query, QueryOptions queryOptions, String regions, int dataRelease) {
query.put(ParamConstants.QueryParams.REGION.key(), regions);
logger.debug("query = " + query.toJson());
logger.debug("queryOptions = " + queryOptions.toJson());
List queries = createQueries(query, regions, ParamConstants.QueryParams.REGION.key());
List queryResults = variantDBAdaptor.nativeGet(queries, queryOptions, dataRelease);
for (int i = 0; i < queries.size(); i++) {
queryResults.get(i).setId((String) queries.get(i).get(ParamConstants.QueryParams.REGION.key()));
}
return queryResults;
}
public CellBaseDataResult getFunctionalScoreVariant(Variant variant, QueryOptions queryOptions, int dataRelease)
throws CellBaseException {
return variantDBAdaptor.getFunctionalScoreVariant(variant, queryOptions, dataRelease);
}
public List> getFunctionalScoreVariant(List variants, QueryOptions options, int dataRelease)
throws CellBaseException {
List> cellBaseDataResults = new ArrayList<>(variants.size());
for (Variant variant: variants) {
if (variant.getType() == VariantType.SNV) {
cellBaseDataResults.add(getFunctionalScoreVariant(variant, options, dataRelease));
} else {
cellBaseDataResults.add(new CellBaseDataResult<>(variant.toString(), 0, Collections.emptyList(), 0));
}
}
return cellBaseDataResults;
}
public List> getPopulationFrequencyByVariant(List variants, QueryOptions queryOptions,
int dataRelease) throws CellBaseException {
return variantDBAdaptor.getPopulationFrequencyByVariant(variants, queryOptions, dataRelease);
}
public CellBaseDataResult getSpliceScoreVariant(Variant variant, String apiKey, int dataRelease) throws CellBaseException {
Set validSources = apiKeyManager.getValidSources(apiKey, ApiKeyLicensedDataUtils.UNLICENSED_SPLICE_SCORES_DATA);
CellBaseDataResult result = spliceDBAdaptor.getScores(variant.getChromosome(), variant.getStart(),
variant.getReference(), variant.getAlternate(), dataRelease);
if (ApiKeyLicensedDataUtils.needFiltering(validSources, ApiKeyLicensedDataUtils.LICENSED_SPLICE_SCORES_DATA)) {
return ApiKeyLicensedDataUtils.filterDataSources(result, validSources);
} else {
return result;
}
}
public List> getSpliceScoreVariant(List variants, String apiKey, int dataRelease)
throws CellBaseException {
Set validSources = apiKeyManager.getValidSources(apiKey, ApiKeyLicensedDataUtils.UNLICENSED_SPLICE_SCORES_DATA);
List> cellBaseDataResults = new ArrayList<>(variants.size());
if (ApiKeyLicensedDataUtils.needFiltering(validSources, ApiKeyLicensedDataUtils.LICENSED_SPLICE_SCORES_DATA)) {
for (Variant variant : variants) {
cellBaseDataResults.add(ApiKeyLicensedDataUtils.filterDataSources(spliceDBAdaptor.getScores(variant.getChromosome(),
variant.getStart(), variant.getReference(), variant.getAlternate(), dataRelease), validSources));
}
} else {
for (Variant variant : variants) {
cellBaseDataResults.add(spliceDBAdaptor.getScores(variant.getChromosome(), variant.getStart(), variant.getReference(),
variant.getAlternate(), dataRelease));
}
}
return cellBaseDataResults;
}
public CellBaseDataResult getFunctionalScoreRegion(List regions, CellBaseQueryOptions options,
int dataRelease)
throws CellBaseException {
Set chunkIdSet = new HashSet<>();
for (Region region : regions) {
chunkIdSet.addAll(variantDBAdaptor.getFunctionalScoreChunkIds(region));
}
return variantDBAdaptor.getFunctionalScoreRegion(new ArrayList<>(chunkIdSet), options, dataRelease);
}
public CellBaseDataResult searchSnp(SnpQuery query) throws CellBaseException {
return snpDBAdaptor.query(query);
}
public CellBaseDataResult startsWithSnp(String id, QueryOptions options, int dataRelease) throws CellBaseException {
return snpDBAdaptor.startsWith(id, options, dataRelease);
}
}
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