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Fluorescence lifetime analysis in SciJava Ops.
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/*-
* #%L
* Fluorescence lifetime analysis in SciJava Ops.
* %%
* Copyright (C) 2024 SciJava developers.
* %%
* Redistribution and use in source and binary forms, with or without
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package org.scijava.ops.flim.impl;
import flimlib.FLIMLib;
import net.imglib2.type.numeric.RealType;
import org.scijava.ops.flim.AbstractFitRAI;
import org.scijava.ops.flim.FitParams;
import org.scijava.ops.flim.FitResults;
import org.scijava.ops.flim.FitWorker;
public class PhasorFit {
/**
* Fits a RAI
*
* @param
* @implNote op names="flim.fitPhasor"
*/
public static class PhasorSingleFitRAI, K extends RealType>
extends AbstractFitRAI
{
@Override
public FitWorker createWorker(FitParams params, FitResults results) {
return new PhasorFit.PhasorFitWorker<>(params, results);
}
}
public static class PhasorFitWorker> extends
AbstractSingleFitWorker
{
private static final int NPARAMOUT = 6;
// Phasor's own buffers
private final float[] z, u, v, tau, tauPhi, tauMod;
public PhasorFitWorker(FitParams params, FitResults results) {
super(params, results);
z = new float[1];
u = new float[1];
v = new float[1];
tauPhi = new float[1];
tauMod = new float[1];
tau = new float[1];
}
@Override
protected void beforeFit() {
for (int i = 0; i < paramBuffer.length; i++) {
// no estimation (+Inf was set by RAHelper#loadData)
// this value makes phasor explode
if (paramBuffer[i] == Float.POSITIVE_INFINITY) {
paramBuffer[i] = 0;
}
}
super.beforeFit();
// setup params
z[0] = paramBuffer[0];
u[0] = paramBuffer[1];
v[0] = paramBuffer[2];
tauPhi[0] = paramBuffer[3];
tauMod[0] = paramBuffer[4];
tau[0] = paramBuffer[5];
}
@Override
protected void doFit() {
final int retCode = FLIMLib.GCI_Phasor(params.xInc, transBuffer,
adjFitStart, adjFitEnd, z, u, v, tauPhi, tauMod, tau, fittedBuffer,
residualBuffer, chisqBuffer);
switch (retCode) {
case -1: // PHASOR_ERR_INVALID_DATA (data == null)
case -2: // PHASOR_ERR_INVALID_WINDOW (nbins < 0)
results.retCode = FitResults.RET_BAD_SETTING;
break;
default: // non-negative: iteration count
results.retCode = retCode >= 0 ? FitResults.RET_OK
: FitResults.RET_UNKNOWN;
break;
}
}
@Override
protected void afterFit() {
// and copies back
paramBuffer[0] = z[0];
paramBuffer[1] = u[0];
paramBuffer[2] = v[0];
paramBuffer[3] = tauPhi[0];
paramBuffer[4] = tauMod[0];
paramBuffer[5] = tau[0];
super.afterFit();
}
@Override
public int nParamOut() {
return NPARAMOUT;
}
@Override
protected AbstractSingleFitWorker duplicate(FitParams params,
FitResults rslts)
{
return new PhasorFitWorker<>(params, rslts);
}
}
}
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