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Genome Damage and Stability Centre SMLM Package Software for single molecule localisation microscopy (SMLM)

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/*- * #%L * Genome Damage and Stability Centre SMLM Package * * Software for single molecule localisation microscopy (SMLM) * %% * Copyright (C) 2011 - 2023 Alex Herbert * %% * This program is free software: you can redistribute it and/or modify * it under the terms of the GNU General Public License as * published by the Free Software Foundation, either version 3 of the * License, or (at your option) any later version. * * This program is distributed in the hope that it will be useful, * but WITHOUT ANY WARRANTY; without even the implied warranty of * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the * GNU General Public License for more details. * * You should have received a copy of the GNU General Public * License along with this program. If not, see * . * #L% */ /** * Provides the engine to perform filtering and fitting of Single Molecule Localisation Microscopy * (SMLM) data. * *

Data is processed as 2D frames from an image source. The engine distributes the input image * frames to workers for analysis. Each worker filters the frames to identify candidate * localisations and then fits the candidates using a Gaussian 2D function. The fits are assessed * using criteria such as localisation precision, Signal-to-Noise Ratio (SNR), drift, and spot * width. Fitting may not process all candidates due to the use of smart stopping criteria based on * the acceptance rate. * * @since 1.0 */ package uk.ac.sussex.gdsc.smlm.engine;