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A Java library for the manipulation of files in common bioinformatics formats using the Hadoop MapReduce framework.
// Copyright (c) 2013 Aalto University
//
// Permission is hereby granted, free of charge, to any person obtaining a copy
// of this software and associated documentation files (the "Software"), to
// deal in the Software without restriction, including without limitation the
// rights to use, copy, modify, merge, publish, distribute, sublicense, and/or
// sell copies of the Software, and to permit persons to whom the Software is
// furnished to do so, subject to the following conditions:
//
// The above copyright notice and this permission notice shall be included in
// all copies or substantial portions of the Software.
//
// THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
// IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
// FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
// AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
// LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
// FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS
// IN THE SOFTWARE.
// File created: 2013-07-26 13:54:32
package org.seqdoop.hadoop_bam.util;
import java.io.IOException;
import java.io.InputStream;
import java.net.URI;
import java.nio.file.Paths;
import htsjdk.samtools.cram.ref.ReferenceSource;
import org.apache.hadoop.conf.Configuration;
import org.apache.hadoop.fs.Path;
import htsjdk.samtools.SAMFileHeader;
import htsjdk.samtools.SamInputResource;
import htsjdk.samtools.SamReaderFactory;
import htsjdk.samtools.ValidationStringency;
import org.seqdoop.hadoop_bam.CRAMInputFormat;
public final class SAMHeaderReader {
/** A String property corresponding to a ValidationStringency
* value. If set, the given stringency is used when any part of the
* Hadoop-BAM library reads SAM or BAM.
*/
public static final String VALIDATION_STRINGENCY_PROPERTY =
"hadoopbam.samheaderreader.validation-stringency";
public static SAMFileHeader readSAMHeaderFrom(Path path, Configuration conf)
throws IOException
{
InputStream i = path.getFileSystem(conf).open(path);
final SAMFileHeader h = readSAMHeaderFrom(i, conf);
i.close();
return h;
}
/** Does not close the stream. */
public static SAMFileHeader readSAMHeaderFrom(
final InputStream in, final Configuration conf)
{
final ValidationStringency
stringency = getValidationStringency(conf);
SamReaderFactory readerFactory = SamReaderFactory.makeDefault()
.setOption(SamReaderFactory.Option.EAGERLY_DECODE, false)
.setUseAsyncIo(false);
if (stringency != null) {
readerFactory.validationStringency(stringency);
}
final ReferenceSource refSource = getReferenceSource(conf);
if (null != refSource) {
readerFactory.referenceSource(refSource);
}
return readerFactory.open(SamInputResource.of(in)).getFileHeader();
}
public static ValidationStringency getValidationStringency(
final Configuration conf)
{
final String p = conf.get(VALIDATION_STRINGENCY_PROPERTY);
return p == null ? null : ValidationStringency.valueOf(p);
}
public static ReferenceSource getReferenceSource(
final Configuration conf)
{
//TODO: There isn't anything particularly CRAM-specific about reference source or validation
// stringency other than that a reference source is required for CRAM files. We should move
// the reference source and validation stringency property names and utility methods out of
// CRAMInputFormat and SAMHeaderReader and combine them together into a single class for extracting
// configuration params, but it would break backward compatibility with existing code that
// is dependent on the CRAMInputFormat.REFERENCE_SOURCE_PATH_PROPERTY.
final String refSourcePath = conf.get(CRAMInputFormat.REFERENCE_SOURCE_PATH_PROPERTY);
return refSourcePath == null ? null : new ReferenceSource(NIOFileUtil.asPath(refSourcePath));
}
}